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D3165-02 11-dATP (Biotin) (Biotin-11-dATP) CAS:

Specifications
References
Grade
Highly Purified
Molecular Formula
C30H45N8O15P3S
Molecular Weight
882.71
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
g-[N-(Biotin-6-amino-hexanoyl)]-7-propargylamino-2’-deoxy-7-deaza-adenosine-5’-triphosphate, Triethylammonium Salt

Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications.

Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.
Spectroscopic Properties
λmax 280nm; ε 12,700
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
≥95%
Concentration
~1mM
Form
Supplied as a liquid in 10mM Tris, pH 7.5. Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
[1. Zammatteo et al. (2005) Unambiguous identification of the expressed MAGE-A genes on a DNA microarray. Clin. Chem. 51(12):2420. [2. Dauwerse et al. (1999) Two-colour FISH detection of the inv(16) in interphase nuclei of patients with acute myeloid leukaemia. Br. J. Haematol. 106(1):111. 3. Vorwerk et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N. Biotechnol. 25(2):142. 4. Beier et al. (2008) Microfluidic primer extension assay. Methods Mol Biol 822:143. 5. Vankoningsloo et al. (2008) Gene expression silencing with ’specific’ small interfering RNA goes beyond specificity - a study of key parameters to take into account in the onset of small interfering RNA off-target effects. FEBS J. 275(11):2738.
USBio References
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