Thymidine 5'-(Tetrahydrogen Triphosphate) Sodium Salt; Thymidine-5’-Triphosphate, Sodium Salt; dTTP; 2'-Deoxythymidine 5'-triphosphate trisodium salt
PCR Grade nucleotides are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
dTTP, Molecular Biology Grade, is a 100mM clear, colorless solution of the sodium salt (pH 8.3).
Synonyms
Thymidine 5'-(Tetrahydrogen Triphosphate) Sodium Salt; Thymidine-5’-Triphosphate, Sodium Salt; dTTP; 2'-Deoxythymidine 5'-triphosphate trisodium salt
Usage
250ul at 100mM = 6,250 standard reactions 1,250ul at 100mM = 31,250 standard reactions
CAS No
18423-43-3 Free Acid: 365-08-2
Appearance
Supplied as a liquid
Quality Control
Functionally tested with Sequenase, Taq DNA Polymerase, T7 DNA Polymerase and Pfu DNA Polymerase.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
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