D6045
CAS Number
9011-18-1Grade
Molecular Biology GradeMolecular Weight
500EU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
4°C Do not freezeDextran Sulfate 50% Solution
Suitable for use in hybridization buffers
Dextran Sulfate (MW > 500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% liquid.
A 50% dextran sulfate solution in water filtered through a 20um membrane and autoclaved prior to bottling in clean, sterilized bottles containing no preservatives. Liquid solution is dark brown from autoclaving.
Appearance
Tan to yellow, slighty turbid liquid
RNase, DNase
None Detected
Water
Molecular Biology Grade, USBio Cat No W0900
Applications
Suitable for use as a hybridization accelerator, specifically when preparing rare sequences by Northern or Southern Blots and the rate of hybridization is very slow.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Product should be warmed to RT prior to use. Do not freeze. Aliquots are stable for 12 month after receipt.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
