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D9845-17A 11-dUTP (Biotin) (5mM) CAS:

Specifications
References
Grade
Highly Purified
Molecular Formula
C28H45N6O17P3S (free acid)
Molecular Weight
862.67
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
g-[N-(Biotin-6-amino-hexanoyl)]-5-aminoallyl-2’-deoxy-uridine-5’-triphosphate, Triethylammonium Salt

Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications.

Applications
Suitable for use in DNA labeling by PCR with taq polymerase, DNA labeling by nick translation with DNase I, DNA labeling by primer extention with Klenow 3’-5’ exo-, 3’ end DNA labeling with terminal deoxynucleotidyl transferase (TdT), cDNA labeling by reverse transcription with Moloney Murine Leukemia Virus Reverse Transcriptase. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Spectroscopic Properties
Wavelength max: 240/289nm Extinction coefficient: 10.7/7.11 mmol-1 cm-1 (Tris-HCl, pH 7.5)
Storage and Stability
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
≥ 95% (by HPLC)
Concentration
1mM-1.1mM
Form
Supplied as a sterile liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Konstantou, et al., Europ. J. Human Genetics 17: 105 (2009). 2. Wen, et al., Biosensors & Bioelectronics 19: 685 (2004). 3. Ried, et al., Proc. Natl. Acad. Sci. USA 89: 1388 (1992). 4. Neumann, et al., Naturwissenschaften 73: 553 (1986). 5. Leary, et al., Proc. Natl. Acad. Sci. USA 80: 4045 (1983). 6. Desselberger, et al., Genetourin Med. 66: 313 (1990).
USBio References
No references available
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