5-Cholestene-3B,20a-diol
An important intermediate in corticosteroid biogenesis.
Solubility
Chloroform, Dichloromethane
Method for Determining Identity
Proton NMR Spectroscopic and Mass Spectroscopic Analysis.
TLC Conditions
SiO2; Hexane: Ethyl Acetate; 2:1; Visualized with AMCS and KMnO4; Single Spot; Rf=0.55. Contains 1.5% Methanol.
Optical Rotation
-54.4 (c=0.2, CHCI3)
Storage and Stability
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.