I1904-84H-ML490
Grade
Affinity PurifiedEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
IgG2b (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
FLISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.
Avidity for rat IgG2b: 4.7 x 10e9 M-1.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.