I7661-02B-ML650
Grade
Affinity PurifiedEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Insulin-Like Growth Factor I (IGF1, IGF I, IGF-I, IBP1, Mechano Growth Factor, MGF, Somatomedia C, Somatomedin C) (MaxLight 650)
Insulin-like Growth Factor IA, IGF-IA, IGF1A, Insulin-like Growth Factor IB, IGF-IB, IGF1B
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Growth Factor I, a 70aa secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGFI exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumor cell growth.
IGFI signals through binding to the type 1 insulinlike growth factor receptor (IGF1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGFI is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
Applications
Suitable for use in FLISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 0.67-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.