Kanamycin Sulfate 50mg/ml Solution
O-3-Amino-3-deoxy-α-D-glucopyranosyl-(16)-O-[6-amino-6-deoxy-α-D-glucopyranosyl-(14)]-2-deoxy-D-streptamine Sulfate; Cantrex; Cristalomicina; Enterokanacin; Kamycin; Kanabristol; Kanamytrex; Kanaqua; Kanasig; Kanatrol; Kanescin; Otokalixin; Resistomycin; Amikacin EP Impurity D Sulfate
Kanamycin sulfate is an antibiotic that belongs to the group of drugs called aminoglycosides. It binds to ribosomal components and inhibits protein synthesis. Kanamycin sulfate appears to prevent bacteria from making their cell walls; thus the cells die. It is used to treat many sensitive gram-negative, some gram-positive bacteria and and mycoplasmas in cell culture. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.
Application
Suitable for use as a selectable marker for cosmid vectors used in transfection of mammalian cells.
Form
Filter sterilized (0.2um) solution in 0.9% saline. Supplied in 20ml amber screw-cap vials.
Appearance
Clear solution
Chromatographic Purity
To pass USP
Crystallinity
To pass USP
Specific Rotation
+107° to +121°
Working Concentration
Cell/Tissue Culture: ~30ug/ml Stringent Plasmids: 10ug/ml Relaxed Plasmids: 50ug/ml
Storage and Stability
Store liquid at 4°C. Stable for for 12 months at 4°C. Further dilutions can be made in assay buffer. Stability at 37°C: 5 days.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
