N5375-74
CAS Number
96736-11-7Grade
Highly PurifiedMolecular Formula
(D) C31H58N12O10 · (F) C19H34N8O8 · (E) C25H46N10O9 · (C) C37H70N14O11 · H2SO4Molecular Weight
-758.86502526306988704EU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
-20°CNourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT, Streptothricin Sulfate)
NTC; Nurseotricin; Nourseotricin; clonNAT; Streptothricin Sulfate
A group of antibiotic substances produced by Streptomyces variants. They are composed of an amino sugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of b-amino group of the b-lysine by Nouresothricin N-acetyltransferase the product of the sat1 or nat1 genes. Nourseothricin is a mixture of four streptothricin compounds: D and F (~90%) as well as C and E (~10%)
Synonyms
NTC; Nurseotricin; Nourseotricin; clonNAT; Streptothricin Sulfate
Molecular Formula
(D) C31H58N12O10 · (F) C19H34N8O8 · (E) C25H46N10O9 · (C) C37H70N14O11 · H2SO4
Molecular Weight
Streptothricin D (758.86), Streptothricin F (502.52), Streptothricin E (630.69), Streptothricin C (887.04)
Form
Supplied as a non-sterile powder. Reconstitute with ddH2O to 100mg/ml and sterile filter. Maximal solubility is 1g/ml.
Purity
Components D&F (HPLC): ~90%; Components C&E (HPLC): ~10%
Biological Activity
~110% standard Nr. 82
Elementary Analysis (Typical)
C: 32.3% H: 5.7% N: 14.0% S: 6.7%
Applications
Suitable for use in selection of recombinant Leishmania strains.
Recommended Dilutions
Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher.
Reconstitution
Reconstitute with ddH2O to 100mg/ml and sterile filter. Maximal solubility is 1g/ml.
Selection
For selection of recombinant Leishmania strains Nourseothricin NTC is added to the growth medium to a final concentration of 100ug/ml.
PubChem Chemical ID
Streptothricin D: 202550, Streptothricin F: 197034
Table 1
* MIC: Minimal inhibitory concentration ** IC50: Concentration inhibiting growth by 50%
Group Species MIC* Selection conc. ug/ml ug/ml Gram-negative bacteria Escherichia coli 2-12 50 Francisella tularensis - 5 Pseudomonas aeruginosa 50 100 Gram-positive bacteria Bacillus subtilis 5 50 Enterococcus faecium 8-256 500 Staphylococcus aureus -12 50 Streptomycetes Streptomyces lividans 6 100 Yeast Candida albicans 200 250-450 Cryptococcus neoformans - 100 Hansenula polymorpha - 100 Pichia pastoris - 100 Saccharomyces cerevisiae 25 100 Filamentous fungi Acremonium chrysogenum - 25 Sordaria macrospora - 50 Ustilago maydis - 75 Protozoa Leishmania tarentolae - 100 Phytomonas serpens - 100 Plasmodium falciparum 75* - Toxoplasma gondii - 500 Plants Arabidopsis thaliana 20 200 Oryza sativa 20 200
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Cox, et al., Infect. Immunity 71: 173 (2003). 2. Gold, et al., Gene 142: 225 (1994). 3. Goldstein, et al., Yeast 15: 1541 (1999). 4. Hamano, et al., J. Biol. Chem. 281: 16,842 (2006). 5. Hentges, et al., Yeast 22: 1013 (2005). 6. Jacobs, et al., Nature Protocols 4: 58 (2009). 7. Ji, et al., J. Antibiot. 60: 739 (2007). 8. Joshi, et al., Gene 156: 145 (1995). 9. Kloti, et al., Selectable marker in plants. United States Patent 6696621 (2004). 10. Kojic, et al., Can. J. Microbiology 46: 333 (2000). 11. Kück, et al., Fungal Genetics Newsletter 53: 9 (2006). 12. Lukeš, et al., Mol. Biochem. Parasitol. 148: 125 (2006). 13. Maier, et al., Appl. Environment. Microbiol. 72: 1878 (2006). 14. McDade, et al., Med. Mycol. 39: 151 (2001). 15. Mamoun, et al., PNAS 96: 8716 (1999). 16. Mandal, et al., Ind. J. Exptl. Biol. 47: 475 (2009). 17. Nagotu, et al., Traffic 9: 1471 (2008). 18. Roemer, et al., Molec. Microbiol. 50: 167 (2003). 19. Shen, et al., Infect. Immun. 73: 1239 (2005). 20. Van, et al., J. Parasitol. 92: 668 (2006). 21. Werner, et al., Antimicrob. Agents Chemother. 45: 3267 (2001).USBio References
No references available