Technical Data

P4200
CAS Number
5625-37-6
Grade
Molecular Biology Grade
MDL Number
MFCD00006159
Molecular Formula
C8H18N2O6S2
Molecular Weight
302.37
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
RT
PIPES Free Acid
1,4-Piperazinediethanesulfonic Acid; 1,4-Piperazinebis(ethanesulfonic acid); NSC 157117; Piperazine-1,4-bis(2-ethanesulfonic Acid); Piperazine-N,N'-bis(2-ethanesulfonic Acid)

PIPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240-700nm, chemically stable, and stable to temperature and concentration changes. PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work.

Synonyms
1,4-Piperazinediethanesulfonic Acid; 1,4-Piperazinebis(ethanesulfonic acid); NSC 157117; Piperazine-1,4-bis(2-ethanesulfonic Acid); Piperazine-N,N'-bis(2-ethanesulfonic Acid)
CAS No
5625-37-6
Molecular Formula
C8H18N2O6S2
Molecular Weight
302.37
Appearance
White powder
Purity
≥99%
Melting Point
>300°C (lit)
Water (KF)
≤0.5%
Absorbance (A280)
≤0.1
Absorbance (A260)
≤0.1
Solubility
1M NaOH: clear, colorless, complete
DNase
None Detected
RNase
None Detected
Protease
None Detected
Storage and Stability
May be stored at RT Stable for 12 months after receipt.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Good, N. E., et al., Biochemistry 5:467 (1966)
USBio References
No references available
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