Technical Data

R2031-71D
Grade
Highly Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
RIG-1 (Retinoic Acid Inducible Gene 1 Protein, RIG-I, Probable ATP-Dependent RNA Helicase DDX58, Dead Box Protein 58, DDX58)

RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.

Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (paraffin): 5ug/ml detects a band at ~84kD in C2C12 cell lysate. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6, is recommended. Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human heart
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Akira S, et al (2006) Pathogen recognition and innate immunity 124:783-801. 2. Yoneyama M et al (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol.; 5:730-737. 3. Sharma S, et al (2003) Triggering the interferon antiviral response through an IKK-related pathway Science 300:1148-51. 4. Alexopoulou L, et al (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3 Nature 413:732-8
USBio References
No references available
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