T5579
CAS Number
4697-14-7,61177-45-5Grade
Highly PurifiedEU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
4°CTimentin (Ticarcillin Disodium/Potassium Clavulanate 15:1)
Timentin; Ticarcillin; MM 14151; [2R-(2α,3Z,5α)]-3-(2-hydroxyethylidene)-7-oxo-4-oxa- 1-azabicyclo[3.2.0]heptane-2-carboxylate disodium/potassium salt
Timentin is a suitable antibiotic for Agrobacterium counterselection and a mixture of two antibiotics: ticarcillin and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase activity which clavulanic acid exhibits activity towards.
Many Agrobacterium isolates are highly susceptible to Timentin. Agrobacterium strains carrying the standard pBR beta-lactamase gene are also Timentin (clavulanic acid) susceptible. Timentin killing of Agrobacterium wild-type strains is three logs greater than with comparable doses of carbenicillin. In plant transformation experiments, 0.1mg/ml Timentin (15:1) is sufficient to counterselect Agrobacterium.
The appropriate dose in tissue culture medium is 0.1 mg/ml (not 0.1 mg/liter) of 15:1 mixture or 0.2 mg/ml of 30:1 mixture. To improve efficacy (of any antimicrobial), solid media are best slightly dried before use. At these concentrations, Timentin exhibits no phytotoxicity to Arabidopsis root cultures.
Ticarcillin Disodium/Potassium Clavulanate =15:1
Synonyms
Timentin; Ticarcillin; MM 14151; [2R-(2α,3Z,5α)]-3-(2-hydroxyethylidene)-7-oxo-4-oxa- 1-azabicyclo[3.2.0]heptane-2-carboxylate disodium/potassium salt
CAS No
4697-14-7,61177-45-5
Assay (HPLC)
≥750ug/mg (ticarcillin, on dried basis) ≥50ug/mg (clavulanic acid, on dried basis)
Appearance
White to off-white to slight yellow powder
Identification (HPLC)
Positive
Solubility
Colorless, clear, complete
Moisture (Karl-Fischer)
≤4.2%
Related Substances
Largest impurity: ≤4% Second impurity: ≤2.5%
Storage and Stability
Store at 4°C in hermetically sealed container, protected from light. Stable for 12 months after receipt at 4°C.
