Technical Data

T5595-4975
Grade
Molecular Biology Grade
EU Commodity Code
38220090
Shipping Temp
Dry Ice
Storage Temp
-70°C
Tissue, Section, Human Adult Normal, Lung (Frozen) HisTek™

Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. The tissues were excised, immediately frozen by liquid nitrogen and then stored in -80ºC. All the tissue are pathologically identified. A single tissue section with 5-10um thickness is mounted on a positively charged glass slide. The slide is fixed and dehydrated with cold acetone for consistent results with in situ hybridization and immunohistochemistry. These sections are an ideal product for rapid cellular localization of RNA and protein expression.

USBio’s frozen tissue section line encompasses a large selection of tissue sections which are good for immunohistochemistry and in situ hybridization. It includes human adult and fetal normal tissues, human diseased and tumor tissues, as well as from mouse, rat, and monkey tissues. All the tissues are diagnosed by a licensed pathologist. Accurate documentation and clinical histories of the tissues can be supplied upon request.
Features
Tissues from a wide variety of hard to obtain tissue sources Suitable for both immunohistochemistry and in situ hybridization Multiple tissues or a single tissue per slide Documentation on tissues' clinical histories available upon request.
Applications
Cellular localization of tissue specific mRNA and protein expression Identification of tumor and disease specific genes, proteins by in situ hybridization, immunohistochemistry, and in situ PCR detection Comparison of the locations of novel genes in different tissues Control slides for immunohistochemistry studies
Quality Control: One of the tissue section slides from each lot was stained with H & E to ensure the quality.
Storage and Stability: Store fresh frozen tissue section slides at -70ºC. Sections are stable for 6 months from the date of receipt under proper storage conditions.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Mark Powzaniuk, Sheila McElwee-Witmer, Robert L. Vogel, Tadashi Hayami, Su Jane Rutledge, Fang Chen, Shun-ichi Harada, Azriel Schmidt, Gideon A. Rodan, Leonard P. Freedman, and Chang Bai. The LATS2/KPM Tumor Suppressor Is a Negative Regulator of the Androgen Receptor. Mol. Endocrinol., Aug 2004; 18: 2011-2023. 2. Naohiko Anzai, Hiroki Miyazaki, Rie Noshiro, Suparat Khamdang, Arthit Chairoungdua, Ho-Jung Shin, Atsushi Enomoto, Shinichi Sakamoto, Taku Hirata, Kimio Tomita, Yoshikatsu Kanai, and Hitoshi Endou. The multivalent PDZ domain-containing protein PDZK1 regulates transport activity of renal urate-anion exchanger URAT1 via its C-terminal. J. Biol. Chem., Aug 2004; 10.1074/jbc.M406724200. 3. Pierfrancesco Tassone, Victor S Goldmacher, Paola Neri, Antonella Gozzini, Masood A Shammas, Kathleen R Whiteman, Linda Hylander, Daniel R Carrasco, Teru Hideshima, Reshma Shringarpure, Jialan Shi, Charles K Allam, John Wijdenes, Salvatore Venuta, Nikhil C Munshi, and Kenneth C Anderson. Cytotoxic activity of the maytansinoid immunoconjugate B-B4-DM1 against CD138+ Multiple Myeloma cells. Blood, Aug 2004; 10.1182/blood-2004-03-0963. 4. Kazuishi Kubota, Kaori Nakahara, Toshiaki Ohtsuka, Shuku Yoshida, Junko Kawaguchi, Yoko Fujita, Yohei Ozeki, Ayako Hara, Chigusa Yoshimura, Hidehiko Furukawa, Hideyuki Haruyama, Kimihisa Ichikawa, Makoto Yamashita, Tatsuji Matsuoka, and Yasuteru Iijima. Identification of 2'-phosphodiesterase, which plays a role in the 2-5A system regulated by interferon. J. Biol. Chem., Jun 2004; 10.1074/jbc.M400089200. 5. Tomoaki Tanaka, Edward T. H. Yeh, and Tetsu Kamitani. NUB1-mediated targeting of the ubiquitin precursor UbC1 for its C-terminal hydrolysis. Eur. J. Biochem., Mar 2004; 271: 972 - 982. 6. Ryoko Iizuka, Katsuyoshi Chiba, and Shinobu Imajoh-Ohmi. A Novel Approach for the Detection of Proteolytically Activated Transglutaminase 1 in Epidermis Using Cleavage Site-Directed Antibodies. J. Invest. Dermatol., Sep 2003; 121: 457 - 464. 7. Daisuke Kobayashi, Takashi Nozawa, Kozue Imai, Jun-ichi Nezu, Akira Tsuji, and Ikumi Tamai. Involvement of Human Organic Anion Transporting Polypeptide OATP-B (SLC21A9) in pH-Dependent Transport across Intestinal Apical Membrane. J. Pharmacol. Exp. Ther., Aug 2003; 306: 703 - 708. 8. Daisuke Kobayashi, Takashi Nozawa, Kozue Imai, Jun-ichi Nezu, Akira Tsuji, and Ikumi Tamai. Involvement of Human Organic Anion Transporting Polypeptide OATP-B (SLC21A9) in pH-Dependent Transport across Intestinal Apical Membrane. J. Pharmacol. Exp. Ther., Apr 2003; 103051300. 9. Takehiko Ogura, Tetsushi Furukawa, Tetsuya Toyozaki, Katsuya Yamada, Ya-Juan Zheng, Yoshifumi Katayama, Haruaki Nakaya, and Nobuya Inagaki. ClC-3B, a novel ClC-3 splicing variant that interacts with EBP50 and facilitates expression of CFTR-regulated ORCC. FASEB J, Apr 2002; 108451. 10. Hisayuki Nomiyama, Kunio Hieshima, Takashi Nakayama, Tomonori Sakaguchi, Ryuichi Fujisawa, Sumio Tanase, Hiroshi Nishiura, Kenjiro Matsuno, Hiroshi Takamori, Youichi Tabira, Tetsuro Yamamoto, Retsu Miura, and Osamu Yoshie. Human CC chemokine liver-expressed chemokine/CCL16 is a functional ligand for CCR1, CCR2 and CCR5, and constitutively expressed by hepatocytes. Int. Immunol., Aug 2001; 13: 1021 - 1029.
USBio References
No references available
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