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T8650 Tris Hydrochloride (Tris HCl) CAS: 1185-53-1

Specifications
References
CAS Number
1185-53-1
Grade
Molecular Biology Grade
Molecular Formula
C4H11NO3•HCl
Molecular Weight
157.6
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
RT
Tris(hydroxymethyl)aminomethane HCl; 2-Amino-2-(hydroxymethyl)-1,3-propanediol HCl; Tromethamine HCl

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. One of the biological buffers.

Synonyms
Tris(hydroxymethyl)aminomethane HCl; 2-Amino-2-(hydroxymethyl)-1,3-propanediol HCl; Tromethamine HCl
CAS No
C4H11NO3•HCl
Molecular Formula
C4H11NO3•HCl
Molecular Weight
157.60
Purity
≥99%
Appearance
White, crystalline powder
pH (1.0M, H2O)
3.5-5.0
pKa (20°C:) 8.3 ± 0.15
Absorbance (260nm)
≤0.01
Absorbance (280nm)
≤0.05
Solubility (1M, H2O)
Colorless, clear, complete
Loss on Drying
≤0.3%
Residue on Ignition
≤0.1%
Melting Point
150-154°C
Heavy Metals (Pb)
≤0.0005%
Iron (Fe)
≤0.005%
Insoluble Matter
≤<0.005%
RNase
None Detected
DNase
None Detected
Protease
None Detected
Storage
RT
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
References
Ma, C. et al., (2013) Electrophoresis, DOI: 10.1002/elps.201200662.
USBio References
US Biological application reference: Ma, C. et al., (2013) Electrophoresis, DOI: 10.1002/elps.201200662.
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