DNA is isolated by proprietary modified guanidine thiocyanate techniques. The DNA is dissolved in 1xTE (10mM Tris, pH 8.0, 1mM EDTA) buffer.
Donor Information
Human, male 77 years old, normal, brain
Quality Control
1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8-2.0, A260/230 is >2.0. (detected in TE, pH 8.0) 2. Plant genomic DNA, FFPE Genomic DNA, and FFPE and Frozen Matched Pair Genomic DNA concentration is determined by pico green measurement. Due to the characteristics of plant and FFPE genomic DNA, the concentration measured by UV is about 4 times more than the true value. While other genomic DNA concentration is determined by UV260 measurement 3. RNase treatment to ensure the RNA contamination is eliminated 4. Hind III digestion test: genomic DNA was successfully digested by Hind III 5. beta-Actin expression was tested by PCR amplification for Genomic DNA or PCR ready genomic DNA
Storage and Stability
Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.