Suitable for use in Northern Blot, RT-PCR, RACE cDNA synthesis, cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection, primer extension, RNA display and Purification of mRNA. Other applications have not been tested.

One 24 year old normal, human adult male, brain: cerebelum (DNase 1 treated)

Total RNA isolated from a wide variety of hard to obtain tissues. Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA Extensive quality control procedures to ensure high quality. High efficiency reverse transcription.

1. The integrity of the RNA is examined by visual inspectiuon for the presence of intact bands of 18s and 28s RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotomer. (A260/280 = 1.8-2.1 detected in 10mM Tris-Cl, pH 7.5). 2. The RNA is treated by DNAse I. It is tested as DNA free RNA by PCR. 3. cDNA synthesis is successfully performed by using this RNA as template.

Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human

Total RNA isolated by modified guanidine thiocyanate techniques.

As Reported

Supplied as a liquid in RNA storage buffer

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.