C2C12 Nuclear Extract, Differentiated (Muscle, Myoblast)
Permanent murine cell lines of fast and slow skeletal myocyte derivation.
Western Blot
Myf-4, Sp1, Sp3, TFIIB
EMSA
E12/E47, Myf-3 (MyoD), Sp1
Storage and Stability
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Purity
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Form
Supplied as a liquid in 20mM Hepes, pH 7.9, 100mM KCl, 1mM MgCl2, 20% glycerol, 0.5mM PMSF, 0.5mM DTT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
