C2272-90D-ML490
Grade
Affinity PurifiedConjugate
MaxLight™490Specificity
Recognizes domain 2 of the mouse cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.Applications
FCEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeBSA Free
YesPreservative Free
YesNotes
Preservative Free
BSA Free
CD22 (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression. CD22 reacts with most B cell leukemias including Hairy Cell Leukemia (HCL) and B cell lymphomas.
Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
