C2445-30-ML490
Grade
Affinity PurifiedConjugate
MaxLight™490Specificity
Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (1).Applications
FC IHC IPEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeBSA Free
YesPreservative Free
YesNotes
Preservative Free
BSA Free
CD101 (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.
Applications: Removal of sodium azide is recommended prior to use in functional assay. Immunohistochemistry: Frozen Immunoprecipitation Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Recommended Secondary Reagents
F(ab')2 Rabbit anti mouse IgG: FITC F(ab')2 Rabbit anti mouse IgG: RPE
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG: HRP conjugate
Recommended Negative Controls
Mouse IgG1 Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
