Technical Data

H1833
Grade
Lysate
Applications
WB
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
HE Pervanadate Control Lysate

Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. Human aortic endothelial cells treated with pervanadate for 30 minutes display a significant increase in tyrosine phosphorylation as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of human aortic endothelial cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1mM) for 30 minutes at 37°C. Cells were lysed in 1% SDS, 1mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Applications
SDS-PAGE/Western blotting 20ml/well. End user should determine optimal quantity for their particular applications and experiments.
Source
Human aortic endothelial cells
Concentration
~1mg/ml
Form
Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1981 Ross, A.H. et al. Nature 294:654.|1988 Wang, J.Y.J. Anal. Biochem 172:1.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net