HeLa Nuclear Extract
Nuclear extract is prepared from human HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins and transcription factors.
Applications
Suitable for use as a positive control for antibodies.
Recommended Usage
Add an equal volume of Laemmli reducing sample buffer to 5-10ul of lysate. Boil for 5 minutes to reduce the preparation. Load 5-10ul (10-20ug) of reduced lysate per lane for immunoblot analysis.
Storage and Stability
For long-term storage, store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in RIPA buffer containing inhibitors.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
