HeLa Nuclear Extract

Grade

Lysate

Applications

EU Commodity Code

38220090

Shipping Temp

Dry Ice

Storage Temp

-70°C

Nuclear extract is prepared from human HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins and transcription factors.

Applications

Suitable for use as a positive control for antibodies.

Recommended Usage

Add an equal volume of Laemmli reducing sample buffer to 5-10ul of lysate. Boil for 5 minutes to reduce the preparation. Load 5-10ul (10-20ug) of reduced lysate per lane for immunoblot analysis.

Storage and Stability

For long-term storage, store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

HeLa cells

Concentration

~2mg/ml

Form

Supplied as a liquid in RIPA buffer containing inhibitors.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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H1838 HeLa Nuclear Extract

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