Multiple Tag is a specially designed fusion protein for use as a positive control for Western blot or Dot blot. The fusion protein is a 40kD protein containing 16 commonly used tags from N-terminus to C-terminus in the order as shown in the table
Tag Sequence Trx MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA T7 CMASMTGGQQMG HSV QPELAPEDPEDC c-Myc EQKLISEEDLC VSV-g YTDIEMNRLGK Glu-Glu CEEEEYMPME V5 GKPIPNPLLGLDST E-tag GAPVPYPDPLEPR Flag-tag DYKDDDDK S-tag KETAAAKFERQHMDS HA YPYDVPDYAC KT3 KPPTPPPEPET E2 GVSSTSSDFRDR AU1 DTYRYI AU5 TDFYLK 6x His HHHHHH
Applications
Suitable for use in Western Blot and Dot Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized product is stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
≥95% by SDS-PAGE under reducing conditions and Coomassie blute staining.
Concentration
~1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from 50mM Tris-HCl, 300mM sodium chloride, 100mM imidazole, pH 8.0. Reconstitute with 200ul of ddH2O.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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