STAT3 Control Cell Extracts

Grade

Lysate

EU Commodity Code

38220090

Shipping Temp

Blue Ice

Storage Temp

-20°C

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. Additionally, Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by tyrosine phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Transcriptional activation seems to be regulated by serine phosphorylation at Ser727, apparently via the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3alpha (86kD) and Stat3beta (79kD) depend on cell type, ligand exposure or maturation stage of the cells. It is notable that Stat3beta lacks the serine phosphorylation site within the C-terminal transcriptional activation domain.

Kit Components

S7976-45A: Phosphorylated Stat3 Control Cell Extracts. Total cell extracts from serum-starved HeLa cells prepared with interferon-alpha treatment, serves as a positive control. Supplied in SDS Sample Buffer (62.5mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50mM DTT)..

S7969-45B: Non-Phosphorylated Stat3 Control Cell Extracts. Total cell extract from serum-starved HeLa cells prepared without treatment serves as a negative control. Supplied in SDS Sample Buffer (62.5mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50mM DTT, 0.01% bromophenol blue).. 1x100ul

Applications

Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions

Western Blot:10ul of phosphorylated and nonphosphorylated Stat1 control cell extracts. Boil 3 minutes prior to first use. Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

HeLa cells

Form

Supplied in SDS Sample Buffer (62.5mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50mM DTT).

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120. 2. Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801–227. 3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-115. 4. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85. 5. Bromberg, J.F. et al. (1999) Cell 98, 295-303. 6. Darnell Jr., J.E. et al. (1994) Science 264, 1415-1421. 7. Ihle, J.N. (1995) Nature 377, 591-594. 8. Wen, Z. et al. (1995) Cell 82, 241-250. 9. Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50. 10. Biethahn, S. (1999) Exp. Hematol. 27, 885-894.

USBio References

No references available

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S7971-45 STAT3 Control Cell Extracts (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

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