Permanent murine cell lines of fast and slow skeletal myocyte derivation.
Nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with PMA (10ng/ml).
Storage and Stability For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Purity
Extracts have been quality control tested by Western blot.
Form
Supplied as a liquid in 20mM Hepes pH 7.5, 400mM NaCl, 20% glycerol, 0.1 mM EDTA, 10mM NaF, 10uM Na2MoO4, 1mM NaVO3, 10mM PNPP, 10mM beta-glycerophosphate, 1mM DTT and protease inhibitors.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.