Tissue, Total Protein, Human Adult Normal, Colon HisTek™

Brand

HisTek™

Grade

Molecular Biology Grade

EU Commodity Code

38220090

Shipping Temp

Dry Ice

Storage Temp

-70°C

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

Applications

Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interaction and Tissue-Specific Expression Identification. Other applications not tested.

Quality Control

1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.

Storage and Stability

For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. Further dilutions can be made in assay buffer.

Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.

Concentration

~5mg/ml

Form

Supplied as a frozen liquid in HEPES, pH7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

Al-Mulla, (2010) “Uses of Microarray Platforms in Cancer”, Microarrays: Volume 2, Applications and Data Analysis, 2nd Edition (J.B. Rampal, Ed.) Springer.

USBio References

US Biological application reference: Al-Mulla, (2010) “Uses of Microarray Platforms in Cancer”, Microarrays: Volume 2, Applications and Data Analysis, 2nd Edition (J.B. Rampal, Ed.) Springer.

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T5595-6473 Tissue, Total Protein, Human Adult Normal, Colon HisTek™

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