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T5595-6555 Tissue, Total Protein, Human Adult Normal, Pancreas HisTek™

Specifications
References
Brand
HisTek™
Grade
Molecular Biology Grade
EU Commodity Code
38220090
Shipping Temp
Dry Ice
Storage Temp
-70°C

Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Applications
• Electrophoresis, Western Blot and Immunoprecipitation • Enzymatic Activity • Protein-DNA Interaction • Protein-Protein Interaction • Tissue-Specific Expression Identification
Features
• Genomic DNA isolated from a wide variety of hard to obtain tissues • Decontamination of polysaccharide and proteoglycan • Extensive quality control procedures ensure high quality genomic DNA • High efficiency in PCR • Documentation of tissues' clinical histories available
Quality Control
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is analyzed by Western Blot using an antibody to GAPDH for total protein, cytoplasmic and universal total protein, histone or nuclear protein, Na/K ATPase for membrane protein, Vimentin for cytoskeleton protein. The expression level is consistent with each lot.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Human
Concentration
~5mg/ml
Form
Supplied as a frozen liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
US Biological application reference: Bai, J. et al., (2005) Cancer Res. 65:2344-2352.
USBio References
US Biological application reference: Bai, J. et al., (2005) Cancer Res. 65:2344-2352.
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