All the protein lysates were prepared from liquid nitrogen fresh frozen tissues. Total protein and universal protein lysates were prepared by a Total Protein Extraction Kit (Cat# P9087).

Human tumor protein matched products include: Primary Pair (PP), or Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor and corresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic genes

1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining, the pattern is consistent with each lot. 2. The isolated protein is analyzed by Western Blot using an antibody to • GAPDH for total protein, cytoplasmic and universal total protein.

Store the protein lysate at -70°C for long term storage. After the protein is thawed, store it at 2-8°C for continuous use. Avoid freeze and thaw cycles. Stable for 6 months.

Supplied in a buffer including HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.