Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Quality Control
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot.
Applications
Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Gel shift assay, Mass spec, Protein-Protein Interaction and Tissue-Specific Expression Identification. Other applications not tested.
Recommended Dilution
Western Blot: 10-20ug per lane for mini gel. Optimal dilutions to be determined by the researcher.
Storage and Stability
For continuous use in a day, store at 4ºC. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.