Total RNAs are available from almost 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection, and RNA differential display. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1/1 between 28S and 18S ribosomal RNA. We guarantee high efficiency reverse transcription.
Features
Total RNA isolated from a wide variety of hard to obtain tissues Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA Extensive quality control procedures to ensure high quality High efficiency reverse transcription Available documentation of tissues' clinical histories (additional cost)
Applications
Northern Blotting, RT-PCR, and RACE cDNA synthesis and cDNA library construction cDNA probe for profiling study in gene expression RNA protection and primer extension RNA display Purification of mRNA
Quality Control
1. The integrity of the RNA is examinend by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresis on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer A260/A280 = 1.8-2 (detected in 10mM tris-Cl, pH 7.5). 2. The RNA is treated by DNase I and is tested as DNA free by PCR. 3. cDNA synthesis is successfully performed by using this RNA as a template. Note: Total RNA from some rare tissues and tumor tissues may not be treated by DNase I
Donor Information
RNA from 5x40-week old female and 5x44-week old male, normal rats.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -70°C. Aliquots are stable for at least 6months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Total RNA isolated by modified guanidine thiocyanate techniques.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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