Universal Protein Lysate, Canine Adult Normal
Universal Protein Lysates
Designed for use as a control for antibody arrays and Western Blot Analysis. Total protein extract is pooled from a collection of high-quality tissue. By using pooled protein from whole tissues you can get better protein representation, homogenous signal intensities, stable expression profiles, and high reproducibility between different lots.
Features
• Broad protein coverage from diversified tissues • Large production scale to minimize lot-to-lot variation
Applications
• Controls for antibody arrays • Controls for Western Blot Analysis
Preparation
All the protein lysates were prepared from liquid nitrogen fresh frozen tissues. Universal protein lysates were prepared by Total Protein Extraction Kit (Cat# P9087).
Quality Control
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining, the pattern is consistent with each lot. 2. The isolated protein is analyzed by Western Blot using an antibody to GAPDH for universal total protein.
Storage and Stability
Store the protein lysate at -70°C for long term storage. After the protein is thawed, store it at 2-8°C for continuous use. Avoid freeze and thaw cycles. Stable for 6 months.
Form
Supplied in a buffer including HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
