Chicken Universal RNA is prepared from major organs of both male and female adult chickens. This total RNA serves as a standard for comparison of gene expression from microarray studies.
Features
Total RNA isolated from a wide variety of hard to obtain tissues Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA Extensive quality control procedures to ensure high quality High efficiency reverse transcription
Quality Control
1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18S and 28S ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/A280 is 1.8-2.0 (detected in 10mM Tris-Cl, pH 7.5). 2. The RNA is treated by DNase I. It is tested as DNA free RNA by PCR. 3. cDNA synthesis is successfully performed by using this RNA as template.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity
Total RNA isolated by modified guanidine thiocyanate techniques.
Form
Supplied as a liquid in RNA Storage Buffer.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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