027667
Kit Type
Sandwich ELISATests
96Sample Volume
100ulSensitivity
26.5pg/mlDetection Method
ColorimetricDetection Range
62.5-4000pg/mlSample Matrix
Serum, plasma, cerebrospinal fluid and other biological fluidsEU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CProstaglandin D2 Synthase, Brain (PGD2S) BioAssay™ ELISA Kit (Human)
Prostaglandin D2 Synthase, Brain (PGD2S) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PGD2S in human serum, plasma, cerebrospinal fluid and other biological fluids.
Detection Range
0.312-20ng/ml
Kit Components
*027667A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027667B: Standard, 2x1 vial 027667C: Standard Diluent, 1x20ml *027667D: Detection Reagent A (green), 1x120ul *027667E: Detection Reagent B (red), 1x120ul 027667F: Assay Diluent A, 1x12ml 027667G: Assay Diluent B, 1x12ml 027667H: TMB Substrate, 1x9ml 027667K: Stop Solution, 1x6ml 027667L: Wash Buffer, 30X, 1x20ml
Precaution
The Stop Solution (027667K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *027667A, *027667B, *027667D and *027667E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
