0.156-800ng/ml

0.61ng/ml

<10%

<12%

*028641A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *028641B: Standard, 2x1vial  028641C: Standard Diluent, 1x20ml *028641D: Detection Reagent A (green), 1x120ul  *028641E: Detection Reagent B (red), 1x120ul  028641F: Assay Diluent A, 1x12ml 028641G: Assay Diluent B, 1x12ml 028641H: TMB Substrate, 1x9ml *028641J: Positive Control, 16.4ng/ml, 1x1vial (Lyophilized) 028641K: Stop Solution, 1x6ml 028641L: Wash Buffer, 30X, 1x20ml

The Stop Solution (028641K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *028641A, *028641B, *028641D, *028641E and *028641J at -20°C. Store all the other components at 4°C. After reconstitution of *028641J, store at 4°C and use within 5 days. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

The control vial is from a porcine serum sample known to contain TRY. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

The control vial contains porcine TRY in porcine serum (lyophilized powder). Note: The porcine source material used in this product is from healthy porcine of SPF standards (specific pathogen-free animals).

16.4ng/ml

Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.