044961
Kit Type
Competitive ELISATests
96Sensitivity
1ng/mlDetection Method
ColorimetricDetection Range
0.001-5ug/mlSample Matrix
Serum, plasma and cell culture supernatantEU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CIrisin Competitive BioAssay™ ELISA Kit (Fibronectin Type III Domain-containing Protein 5, Fibronectin Type III Repeat-containing Protein 2, FNDC5)
Fibronectin Type III Domain-containing Protein 5, Fibronectin Type III Repeat-containing Protein 2, FNDC5
The Irisin Competitive ELISA Kit is to be used for the in vitro quantitative determination of irisin in serum, plasma and cell culture supernatant of human origin. It should also work for the in vitro quantitative determination of irisin in mouse, rat and monkey biological samples. This ELISA Kit is for research use only.
Principle
This assay is a competitive Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of irisin in human biological fluids (plasma, serum, cell culture supernatant). A polyclonal antibody recognizing native irisin reacts with a series of predetermined recombinant irisin standard proteins or samples under competition in the irisin-coated plate. Their relative reactivity is plotted with that of the standard proteins.
Expected Values
Irisin levels range in human plasma and serum from 0.2-2ug/ml.
Sensitivity (Limit of Detection)
1ng/ml
Specificity
This ELISA is specific for the measurement of natural and recombinant irisin in human samples. It should also work in mouse, rat and monkey biological samples. It does not cross-react with FNDC4, human adiponectin, human Nampt, human RBP4, human clusterin, human leptin, human vaspin, human GPX3, human resistin, human ACE2, human lipocalin-2, human ANGPTL3, human ANGPTL6, human DNER, human DLK1, human calreticulin, human IL-33, mouse Nampt, mouse clusterin, mouse vaspin, mouse resistin.
Kit Components
044961A: Microtiter Strips, 1x96wells 044961B: Wash Buffer, 10X, 2x30ml 044961C: Diluent, 10X, 2x30ml 044961D: Detection Antibody, 1x30ul 044961E: IgG (HRP) 100X, 1x150ul *044961F: Standard, 1x5ug 044961H: TMB Solution, 1x12ml 044961J: Stop Solution, 1x12ml
Storage and Stability
Store *044961F powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
