This assay is a competitive Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of irisin in human biological fluids (plasma, serum, cell culture supernatant). A polyclonal antibody recognizing native irisin reacts with a series of predetermined recombinant irisin standard proteins or samples under competition in the irisin-coated plate. Their relative reactivity is plotted with that of the standard proteins.

Irisin levels range in human plasma and serum from 0.2-2ug/ml.

0.001-5ug/ml

1ng/ml

This ELISA is specific for the measurement of natural and recombinant irisin in human samples. It should also work in mouse, rat and monkey biological samples. It does not cross-react with FNDC4, human adiponectin, human Nampt, human RBP4, human clusterin, human leptin, human vaspin, human GPX3, human resistin, human ACE2, human lipocalin-2, human ANGPTL3, human ANGPTL6, human DNER, human DLK1, human calreticulin, human IL-33, mouse Nampt, mouse clusterin, mouse vaspin, mouse resistin.

044961A: Microtiter Strips, 1x96wells 044961B: Wash Buffer, 10X, 2x30ml 044961C: Diluent, 10X, 2x30ml 044961D: Detection Antibody, 1x30ul 044961E: IgG (HRP) 100X, 1x150ul *044961F: Standard, 1x5ug 044961H: TMB Solution, 1x12ml 044961J: Stop Solution, 1x12ml

Store *044961F powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.