045168
Kit Type
ELISATests
96Sensitivity
60pg/mlDetection Method
ColorimetricDetection Range
0.125-8ng/mlSample Matrix
SerumEU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°CProgranulin BioAssay™ ELISA Kit (Mouse) (Proepithelin, PEPI, PC Cell-derived Growth Factor)
Proepithelin, PEPI, PC Cell-derived Growth Factor
Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. New cutting-edge publications have shown that progranulin might be a biomarker not only for FTLD, but also for other types of Alzheimer's disease (AD). This new data even supposes the potential of progranulin to be a biomarker for MCI (Mild Cognitive Impairment), which would be a hallmark in AD research. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.
Sample Type
Cell Culture Supernatant, Serum
Intended Use
The Progranulin (mouse) ELISA Kit is to be used for the in vitro quantitative determination of mouse progranulin in serum and cell culture supernatant. This ELISA Kit is for research use only.
Specificity
Detects mouse progranulin. Does not cross-react with human progranulin.
Test Principle
This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of progranulin in the samples.
Kit Components
1 plate coated with mouse progranulin Antibody Wash Buffer 10X, 2x30ml ELISA Buffer 10X, 2x30ml Detection Antibody, 1x20ul HRP Labeled Streptavidin (lyophilized), 1x2ug Standard (lyophilized), 1x16ng TMB Substrate Solution, 1x12ml Stop Solution, 1x12ml
Storage and Stability
Store kit at 4°C, stable for 12 months after receipt. After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use.
