132577-ML550
Conjugate
MaxLight™550Specificity
Recognizes human RICTOR.Source Antibody
humanPurity
Purified by Protein A affinity chromatography.Accession Number
NM_152756, NP_689969EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
RICTOR (Rapamycin-insensitive Companion of mTOR, AVO3 Homolog, hAVO3, KIAA1999, mAVO3, PIA) (MaxLight 550)
DKFZp686B11164, KIAA1999, MGC39830
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Sandwich FLISA: The detection limit for recombinant GST tagged RICTOR is ~0.03ng/ml as a capture antibody. Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml detects RICTOR using immunoperoxidase staining on formalin-fixed paraffin-embedded human small Intestine. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
