143743
Gene ID
1520Kit Type
Sandwich ELISATests
96Sensitivity
< 10 pg/mlDetection Method
ColorimetricDetection Range
62.5pg/ml-4000pg/mlSample Matrix
cell culture supernates, serum and plasma (heparin, EDTA)EU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CCathepsin S BioAssay™ ELISA Kit, Human
Sandwich ELISA quantitative detection kit for Human Cathepsin S in cell culture supernates, serum and plasma (heparin, EDTA).
Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization (FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Assay Range
62.5pg/ml-4000pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Kit Components
96-well plate pre-coated with anti-Cathepsin S, Human Lyophilized recombinant Cathepsin S, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Driessen, C., Bryant, R. A., Lennon-Dumenil, A. M., Villadangos, J. A., Bryant, P. W., Shi, G. P., Chapman, H. A., Ploegh, H. L. Cathepsin S controls the trafficking and maturation of MHC class II molecules in dendritic cells. J. Cell Biol. 147: 775-790, 1999.|2. Riese, R. J., Shi, G.-P., Villadangos, J., Stetson, D., Driessen, C., Lennon-Dumenil, A.-M., Chu, C.-L., Naumov, Y., Behar, S. M., Ploegh, H., Locksley, R., Chapman, H. A. Regulation of CD1 function and NK1.1+ T cell selection and maturation by cathepsin S. Immunity 15: 909-919, 2001.|USBio References
No references available