143890
Gene ID
25325Kit Type
Sandwich ELISATests
96Sensitivity
< 4 pg/mlDetection Method
ColorimetricDetection Range
31.2pg/ml-2000pg/mlSample Matrix
cell culture supernates, serum and plasma (EDTA)EU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CIL-10 BioAssay™ ELISA Kit, Rat
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
Intended Use
IL-10 BioAssay™ ELISA Kit, Rat is a sandwich ELISA for quantitative detection of rat IL-10 in cell culture supernates, serum and plasma (EDTA).
Specificity
Natural and recombinant rat IL-10
Crossreactivity
No detectable crossreactivity with other relevant proteins
Kit Components
143890A: Microtiter Strips, 1x96 wells 143890B: IL-10 Standard, 2x10ng 143890C: Anti-rat IL-10 (Biotin), 130ul
USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution) 143697: Sample Diluent Buffer, 1x30ml 143698: Antibody Diluent Buffer, 1x12ml 143699: ABC Diluent Buffer, 1x12ml 143700: TMB Color Developing Agent, 1x10ml 143701: TMB Stop Solution, 2N H2SO4, 1x10ml
Assay Summary
1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS. 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5times with 0.01M TBS. 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes. 5. Add TMB stop solution and read.
Storage and Stability
Store *143890B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
