143903
Gene ID
3606Kit Type
Sandwich ELISATests
96Sensitivity
< 1 pg/mlDetection Method
ColorimetricDetection Range
15.6pg/ml-1000pg/mlSample Matrix
cell culture supernates, serum and plasma (heparin, EDTA)EU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CIL-18 BioAssay™ ELISA Kit, Human
Sandwich ELISA quantitative detection kit for Human IL-18 in cell culture supernates, serum and plasma (heparin, EDTA).
Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
Assay Range
15.6pg/ml-1000pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Kit Components
96-well plate pre-coated with anti-IL-18, Human Lyophilized recombinant IL-18, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Okamura, H.; Tsutsui, H.; Komatsu, T.; Yutsudo, M.; Hakura, A.; Tanimoto, T.; Torigoe, K.; Okura, T.; Nukada, Y.; Hattori, K.; Akita, K.; Namba, M.; Tanabe, F.; Konishi, K.; Fukuda, S.; Kurimoto, M. Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378: 88-91, 1995. |2. Reddy, P.; Teshima, T.; Kukuruga, M.; Ordemann, R.; Liu, C.; Lowler, K.; Ferrara, J. L. M. Interleukin-18 regulates acute graft-versus-host disease by enhancing Fas-mediated donor T cell apoptosis. J. Exp. Med. 194: 1433-1440, 2001. |3. Mallat, Z.; Corbaz, A.; Scoazec, A.; Besnard, S.; Leseche, G.; Chvatchko, Y.; Tedgui, A. Expression of interleukin-18 in human atherosclerotic plaques and relation to plaque instability. Circulation 104: 1598-1603, 2001. |USBio References
No references available