143944
Gene ID
25818Kit Type
Sandwich ELISATests
96Sensitivity
< 10 pg/mlDetection Method
ColorimetricDetection Range
78pg/ml-5000pg/mlSample Matrix
cell culture supernates, serum, plasma (heparin, EDTA) human milk and salivaEU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CKLK5 BioAssay™ ELISA Kit, Human
Sandwich ELISA quantitative detection kit for Human KLK5 in cell culture supernates, serum, plasma (heparin, EDTA) human milk and saliva.
Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
Assay Range
78pg/ml-5000pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Kit Components
96-well plate pre-coated with anti-KLK5, Human Lyophilized recombinant KLK5, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Kim, H., Scorillas, A., Katsaros, D., Yousef, G. M., Massobrio, M., Fracchioli, S., Piccinno, R., Gordini, G., Diamandis, E. P. Human kallikrein gene 5 (KLK5) expression is an indicator of poor prognosis in ovarian cancer. Brit. J. Cancer 84: 643-650, 2001.|2. Brattsand M, Stefansson K, Lundh C, et al. (2005). "A proteolytic cascade of kallikreins in the stratum corneum". J. Invest. Dermatol. 124 (1): 198–203.|USBio References
No references available