144013
Gene ID
5228Kit Type
Sandwich ELISATests
96Sensitivity
< 1 pg/mlDetection Method
ColorimetricDetection Range
15.6pg/ml-1000pg/mlSample Matrix
cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urineEU Commodity Code
38220000UN DOT Shipping
UN2796 PGIIShipping Temp
Blue IceStorage Temp
4°C/-20°CPLGF BioAssay™ ELISA Kit, Human
Sandwich ELISA quantitative detection kit for Human Placental Growth Factor in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.
The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Assay Range
15.6pg/ml-1000pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Kit Components
96-well plate pre-coated with anti-PLGF, Human Lyophilized recombinant PLGF, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Maglione, D.; Guerriero, V.; Viglietto, G.; Delli-Bovi, P.; Persico, M. G. : Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor. Proc. Nat. Acad. Sci. 88: 9267-9271, 1991.|2. Autiero, M.; Waltenberger, J.; Communi, D.; Kranz, A.; Moons, L.; Lambrechts, D.; Kroll, J.; Plaisance, S.; De Mol, M.; Bono, F.; Kliche, S.; Fellbrich, G.; and 16 others : Role of PlGF in the intra- and intermolecular cross talk between the VEGF receptors Flt1 and Flk1. Nature Med. 9: 936-943, 2003.|3. PLGF stimulated angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF. Luttun, A.; Tjwa, M.; Moons, L.; Wu, Y.; Angelillo-Scherrer, A.; Liao, F.; Nagy, J. A.; Hooper, A.; Priller, J.; De Klerck, B.; Compernolle, V.; Daci, E.; and 10 others : Revascularization of ischemic tissues by PlGF treatment, and inhibition of tumor angiogenesis, arthritis and atherosclerosis by anti-Flt1. Nature Med. 8: 831-840, 2002.|USBio References
No references available