Technical Data

171880
Kit Type
Kinetic
Tests
96
Detection Method
Fluorescent
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C
HDAC2 Kinetic BioAssay™ Kit

The Kinetic HDAC2 BioAssay™ Kit is a complete assay system designed to measure histone deacetylase 2 (HDAC2) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC2 reaction on a microtiter plate is needed and then the HDAC2 activity level can be measured kinetically using a fluorescence reader.

Applications
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Components
HDAC2 human recombinant enzyme, 1x5ug Fluorogenic HDAC substrate (5mM), 1x50ul 10x HDAC Developer, 1x1ml Developer Dilution Buffer, 1x10ml SAHA (1mM), 1x20ul HDAC Assay Buffer, 1x20ml BSA (1mg/ml), 1x1ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89.
USBio References
No references available
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