Technical Data

171887
Kit Type
Assay
Tests
100
Detection Method
Fluorescent
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C
HDAC6 Fluorogenic BioAssay™ Kit

The Fluorogenic HDAC6 BioAssay™ Kit is a complete assay system designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Applications
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Components
HDAC6 human recombinant enzyme, 1x9ug Fluorogenic HDAC substrate (5mM), 1x50ul 2x HDAC Developer (contains Trichostatin A) (50um), 1x6ml Trichostatin A (200um), 1x100ul HDAC assay buffer, 1x10ml black, low binding NUNC black microtiter plate, 1x1plate
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89. 2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar;6(3): 238-243.
USBio References
No references available
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