Technical Data

171905
Kit Type
AlphaLISA
Tests
384
Detection Method
Streptavidin-Biotin
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C
HSP90beta (C-Terminal) Inhibitor Screening BioAssay™ Kit

The HSP90beta (C-Terminal) Inhibitor Screening BioAssay™ Kit is designed to measure the inhibition of HSP90beta binding to its protein target PPID (also known as Cyclophilin D). The assay kit comes in a convenient AlphaLISA® format, with enough HSP90beta (527-724), assay buffer, detection buffer, and purified GST-tagged PPID to perform a total of 384 enzyme reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing HSP90beta, PPID, and an inhibitor of choice is incubated for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Applications
Useful for screening inhibitors of HSP90beta and for HSP90beta binding assays.
Components
HSP90beta (C-terminal), Biotin-labeled, 1x100ug PPID, GST-tag, 1x100ug 3x HSP90 Assay Buffer 2 3x HSP90 Detection Buffer
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Allan, R.K. et al. J. Biol.Chem 2006 281(11): 7161-71
USBio References
No references available
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