Technical Data

172373
Kit Type
Kinetic
Tests
100
Detection Method
Fluorescent|FL530/585nm
Detection Range
0.6-500um
Sample Matrix
food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
Isocitrate BioAssay™ Kit (Isocitrate Acid)

Isocitrate (Isocitrate Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. The isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex/em= 530/585nm, is proportional to the isocitrate concentration in the sample.

Intended Use
For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Applications
Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Range
0.6-500um for 10 min reaction.
Detection limit
0.6um
Kit Components
Assay Buffer, 1x10ml NADP, 1x500ul Standard, 1x1ml Enzyme A, 1x120ul Enzyme B, 1x120ul Probe, 1x750ul
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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