Technical Data

172384
Kit Type
Kinetic
Tests
100
Sensitivity
0.1U/L
Detection Method
OD565nm
Detection Range
0.1-125U/L
Sample Matrix
blood, cell/tissue lysates.
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
Sorbitol Dehydrogenase BioAssay™ Kit (SDH)

Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. The non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The increase in absorbance at 565nm is directly proportional to the enzyme activity.

Intended Use
For quantitative determination of Sorbitol Dehydrogenase enzyme activity.
Applications
SDH activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Range
0.1-125U/L for 12 min reaction.
Detection limit
0.1U/L
Kit Components
Assay Buffer, 1x10ml Substrate, 1x250ul NAD/MTT Solution, 1x1ml Diaphorase, 1x120ul Calibrator, 1x10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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