Technical Data

298464
Kit Type
Assay
Tests
96
Detection Method
Streptavidin-Biotin
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C/-70°C
SUV39H1, Chemiluminescent, BioAssay™ Kit (KMT1A, Suppressor Of Variegation 3-9 Homolog 1 (Drosophila), Su(Var)3-9 Homolog 1)
Intended Use
The SUV39H1 Chemiluminescent BioAssay™ Kit is designed to measure SUV39H1 activity for screening and profiling applications.
Test Principle
The key to the SUV39H1 Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibodys followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Applications
Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.
Kit Components
Strip plate module precoated with histone substrate, 1 plate, 12x8-well strips SUV39H1 human enzyme, 1x10ug S-adenosylmethionine, 100um, 1x250ul Primary antibody 1, 1x100ul Secondary HRP-labeled antibody 1, 1x10ul HMT assay buffer 1, 4x, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate A, (transparent bottle), 1x6ml HRP chemiluminescent substrate B, (brown bottle), 1x6ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Dillon S.C., et al. 2005. Genome Biology 6:227.
USBio References
No references available
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