100ug of antibody at 0.9-1.0mg/ml

Complete and purified conjugation in 1hour with 15 hands on time: >90% yield

Everything included spin, transfer, incubate, spin, spin, done.

No buffers, no organic solvents, no calculations Simple protocol using just a microcentrifuge and a Nanodrop or UV spec

Incorporates the optimal number of dye per antibody

Pure product is produced, all excess label is removed. Exclusive to MaxLight™ kits

Multiple antibodies can be modified simultaneously with different labels

1. Prepare Antibody (~100ug@~1mg/ml) 2. Prepare Desalting Column (Blue Cap), add antibody, centrifuge (2min) 3. Add Antibody to Labeling Column (Clear Cap), vortex, incubate (1hr, dark, RT) 4. Prepare 2 Purification Columns (Red Cap), add labeled antibody, centrifuge (2min) 5. Repeat (add antibody to Purification Column #2 (Red Cap), centrifuge (2min) 6. Flow-through is final labeled antibody, free of unconjugated dye.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

MaxLight™ Biotin (PEG4) MaxLight™ HRP MaxLight™ FITC MaxLight™405 (Violet: compare to AlexaFluor®405, PacificBlue™, Brilliant™ Violet 421) MaxLight™490 (Blue-Green: compare to DyLight™488, AlexaFluor®488) MaxLight™550 (Yellow-Green: compare to AlexaFluor®546, 555, DyLight™549 , Cy3™, TRITC) MaxLight™650 (Far-IR: compare to AlexaFluor®647, DyLight™649, Cy5™) MaxLight™750 (Near IR: compare to DyLight™755)

Specifications: Abs Em ε MaxLight™ Biotin — — — MaxLight™ HRP — — — MaxLight™ FITC 494 520 73,000 MaxLight™405 400 423 32,000 MaxLight™490 493 518 70,000 MaxLight™550 550 568 150,000 MaxLight™650 646 674 120,000 MaxLight™750 655 676 250,000